Effects of environmental estrogens on tumor necrosis factor -mediated

["Kenichi Azuma" <azuma@kcn.ne.jp>]

Dear colleagues


Here is a article of Dr McLachlan.

http://carcin.oupjournals.org/cgi/content/abstract/20/11/2057

Reference: Carcinogenesis, Vol. 20, No. 11, p2057-2061, November 1999
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Cancer Biology

"Effects of environmental estrogens on tumor necrosis factor -mediated
apoptosis in MCF-7 cells"

Matthew E Burow1,2,3,4, Yan Tang2,4, Bridgette M. Collins-Burow11,3,
Stanislaw Krajewski6, John C. Reed6, John A. McLachlan1,2,3,5 and Barbara S
Beckman1,2,3,4,7

1 Molecular and Cellular Biology Program,
2 Department of Pharmacology,
3 Tulane-Xavier Center for Bioenvironmental Research,
4 Tulane Cancer Center and
5 Department of Environmental Health Sciences, Tulane University School of
Medicine, New Orleans, LA 70112 and
6 The Burnham Institute, La Jolla, CA 92037-1062, USA

Environmental estrogens represent a class of compounds which have been shown
to mimic the effects or activity of the naturally occurring ovarian hormone
17s-estradiol. Given the role of 17s-estradiol in cell survival in a number
of systems, we wished to determine if environmental estrogens protect MCF-7
cells from apoptosis. Here we demonstrate that the organochlorine pesticides
o, p' DDT and alachlor, like 17s-estradiol, have the ability to suppress
tumor necrosis factor  (TNF)-induced apoptosis in estrogen receptor
(ER)-positive MCF-7 breast carcinoma cells. These compounds, however, did
not affect TNF-induced apoptosis of the ER-negative MDA-MB-231 cell line.
The ability of these compounds to suppress apoptosis in MCF-7 cells was
correlated with an ER-dependent increase in Bcl-2 expression. Taken together
these results demonstrate that estrogenic organochlorine pesticides like o,
p' DDT and alachlor may partially mimic the primary endogenous estrogen,
17s-estradiol, and function to suppress apoptosis in ER-responsive cells.


Abbreviations: alachlor,
2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide; o,p' DDT,
1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) ethane; DMEM,
Dulbecco's modified Eagle's medium; endosulfan II
(3,5as,6s,9s,9as)-6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-metha
no-2,4,3-benzodioxathiepin 3-oxide: ER, estrogen receptor; ERE, estrogen
response element; FBS, fetal bovine serum; TNF, tumor necrosis factor .

7 To whom correspondence should be addressed at: Tulane University Medical
Center, Department of Pharmacology SL-83, 1430 Tulane Avenue, New Orleans,
LA 70112, USA Email: bbeckman@tmcpop.tmc.tulane.edu

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Copyright c 1999 by Oxford University Press.



Regards,

Kenichi Azuma, Japan

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Kenichi Azuma
http://www.kcn.ne.jp/~azuma/                               (In Japanese)
http://www.kcn.ne.jp/~azuma/English/english_index.htm (In English)
E-mail: azuma@kcn.ne.jp
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